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wormlab data  (GraphPad Software Inc)

 
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    GraphPad Software Inc wormlab data
    Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the <t>WormLab</t> automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.
    Wormlab Data, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wormlab data/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    wormlab data - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Expression and function of Caenorhabditis elegans UNCP-18, a paralog of the SM protein UNC-18"

    Article Title: Expression and function of Caenorhabditis elegans UNCP-18, a paralog of the SM protein UNC-18

    Journal: Genetics

    doi: 10.1093/genetics/iyad180

    Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the WormLab automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.
    Figure Legend Snippet: Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the WormLab automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.

    Techniques Used: Mutagenesis, Transmission Assay, Control, Standard Deviation, Produced, Over Expression



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    wormlab data  (GraphPad Software Inc)
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    GraphPad Software Inc wormlab data
    Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the <t>WormLab</t> automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.
    Wormlab Data, supplied by GraphPad Software Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/wormlab data/product/GraphPad Software Inc
    Average 90 stars, based on 1 article reviews
    wormlab data - by Bioz Stars, 2026-04
    90/100 stars
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    Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the WormLab automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.

    Journal: Genetics

    Article Title: Expression and function of Caenorhabditis elegans UNCP-18, a paralog of the SM protein UNC-18

    doi: 10.1093/genetics/iyad180

    Figure Lengend Snippet: Analysis of an uncp-18 null allele and genetic interactions with unc-18 . a) Quantification of locomotory behavior using the WormLab automated multiworm tracking system reveals reveal no significant locomotory defects of uncp-18 ( syb6377 ) mutant animals. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. These data have been collected on 2 different days and were then combined. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. **** P < 0.0001. n ≥ 10 for all genotypes. b) Aldicarb assays reveal no synaptic transmission defects in uncp-18 ( syb6377 ) mutant animals. Tests with aldicarb resistant ric-4( md1088 ) were done as control. Two-way ANOVA followed by Tukey's multiple comparisons test was done. *** P < 0.001, **** P < 0.0001. n = 3 independent experiments (25 animals per independent experiment). c) Reduced brood size of uncp-18 ( syb6377 ) mutant compared to P0 from the uncp-18 ( syb6377 ) with WT males cross and WT animals. The progeny of at least 20 hermaphrodites was scored at 20°C for each genotype until the worms stop laying progeny, starting with early adulthood. Error bars indicate the standard deviation across 2 separate individual replicates. Stasticial significance was calculated using the Kruskal-Wallis test * P < 0.05, **** P < 0.0001. d) uncp-18 ( syb6377 ) mutant animals produce unfertilized eggs, assessed by quantifying the percentage of eggs, laid by 10 single worms, that hatched to produced viable progeny. Statistics were calculated using Student's t-test. * P < 0.05 e) Overexpression of uncp-18 partially rescues the locomotory defects of unc-18 null mutants, measured with the WormLab automated multiworm tracking system. Each dot represents the indicated feature value for a single animal with the mean ± SEM indicated. The n corresponds to the combination of results collected on 2 different day, explaining the variability of the locomotory data of panel a. For comparisons, the Kruskal–Wallis test followed by Dunn's multiple comparisons was used. ** P < 0.01, *** P < 0.001, **** P < 0.0001. n ≥ 10 for all genotypes. f, g) unc-18 ( e81 ); uncp-18 ( syb6377 ) double null mutants are embryonic lethal. Analysis of the viability of the offspring of 4 single unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers shows that about ¼ of the offspring are inviable (f). g) A representative image of an arrested, disorganized animal (red arrowhead), seen alongside viable progeny at various stages of embryonic development. See also . Note that unlike uncp-18 null mutants, which do not have any maternal contribution of uncp-18 (hence resulting in apparently partially penetrant fecundity effects), the progeny of unc-18 ( e81 ); uncp-18 ( syb6377 )/+ mothers do have wild-type uncp-18 maternal gene dosage.

    Article Snippet: WormLab data were exported to Prism (GraphPad), and statistical significance between each group was calculated using Kruskal–Wallis test followed by Dunn's multiple comparisons.

    Techniques: Mutagenesis, Transmission Assay, Control, Standard Deviation, Produced, Over Expression